作者: Bo Yu, Ying Zhou, Lihua Dou, Yunyun Li, Zhengwen Huang

Abstract: A new xanthene dye, namely ImX, has been facilely prepared by reaction of 4-(1H-Imidazol-1-yl)benzaldehyde with N, N-diethyl-3-aminophenol in concentrated propionic acid, and then treated by p-chloranil. ImX presents the maximum absorption and emission band centered at 562 nm and 583 nm in water, respectively. Fluorescent spectra investigations demonstrate that ImX shows viscosity-selective fluorescent response and emission enhancement when the solvent viscosity increases from 1.1 cp. (water) to 1248 cp. (98 % glycerol). In addition, this viscosity-selective fluorescence response covers a wide pH range from 2.5 to 10.0. More significantly, ImX demonstrates low cytotoxicity and can be employed as tracer for the detection of Monensin-triggered viscosity enhancement by cell imaging.

Keywords: Xanthene, Viscosity, Fluorescence imaging

DOI: 10.1007/s10895-021-02705-4