作者: Jinyao. Hu, Jian Liu, Dongzhou Deng, Wuxian Yan

Abstract: Solanum xanthocarpum (yellow-berried nightshade) is a common weed in southwest China. In august 2014, two S. xanthocarpum plants with leaves displaying ringspots were observed near Mianyang (Sichuang province, China). Total RNAs was extracted from symptomatic and symtomless leaves using Trizol (Invitrogen, USA), and first strand cDNA synthesis was primed with M14T (5’-GTTTTCCCAGTCA CGAC(T)18-3’) by SuperScript III Reverse Transcriptase(Invitrogen, USA). RT-PCR was performed using the potyvirus universal primers Sprimer (5’-GGX AAY AAY AGY GGX CAZ CC-3’, X=A, G, C or T; Y=T or C; Z=A or G) and M4 (5’-GTT TTC CCA GTC ACG AC-3’) (Chen et al., 2001). A 1.6 kb DNA fragment was amplified from symptomatic but not from symptomless leaf samples. The amplified products were purified, cloned into the PMD19-T vector (TaKaRa, China) and sequenced. Sequencing results confirmed the presence of Chilli ringspot virus (ChiRSV) (GenBank accession No. KP310866) with 99% (JN008909.1) and 91% (DQ925438) nucleotide identity with comparable sequences of other ChiRSV isolates available in GenBank. To the best of our knowledge, this is the first report of ChiRSV on S. xanthocarpum in China.

Keywords: biological control agents, genes, genetic markers, geographical distribution, natural enemies, new geographic records, nucleotide sequences, plant pathogens, plant viruses reverse transcriptase PCR

DOI: 10.4454/JPP.V97I1.024